FastQCFastQC Report
Wed 8 May 2024
EGAF00007883981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007883981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836013
Total Bases286.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC307941.0858201284690867Illumina Single End PCR Primer 1 (96% over 33bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC49320.1739061139705636No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC37600.13258049240253833No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT31590.11138877007968581No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC29110.10264409930419924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)