FastQCFastQC Report
Wed 8 May 2024
EGAF00007883983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007883983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906066
Total Bases293.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC306911.0561012723042078Illumina Single End PCR Primer 1 (96% over 33bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC50080.17232919004592462No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC37930.13052009142256232No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT33020.11362439806941754No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC30050.10340439618370678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)