FastQCFastQC Report
Wed 8 May 2024
EGAF00007884085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007884085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276362
Total Bases229.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC188440.8278120966700376Illumina Single End PCR Primer 1 (96% over 33bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT53160.23353051931107618No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC29320.12880200952221132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)