FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007885664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007885664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477541359
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC49562671.0378717793949235Illumina Single End PCR Primer 1 (96% over 31bp)
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT15998450.3350170555593699No Hit
CATAATGGCGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA11185770.2342366747756397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)