FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007888214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007888214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488394283
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG121810432.4941002431840507TruSeq Adapter, Index 3 (97% over 35bp)
CCCGGATGTACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT22193470.4544170718722357No Hit
CCCGGATGTACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA12098990.24772996779735035No Hit
CCCGGATGTACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC6784400.13891235495891338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)