FastQCFastQC Report
Fri 3 May 2024
EGAF00007888215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007888215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488394283
Total Bases73.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC115316642.361138203577211Illumina Single End PCR Primer 1 (96% over 33bp)
CCCGGATGTACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT18326630.37524251691537513No Hit
CCCGGATGTACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA14626660.2994846686196775No Hit
CCCGGATGTACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC5976430.12236895901584499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)