FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007888216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007888216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477079290
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG116995602.452330303417698TruSeq Adapter, Index 3 (97% over 35bp)
CCCGGATGTACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT22194340.4652128160918492No Hit
CCCGGATGTACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA12217650.2560926507625179No Hit
CCCGGATGTACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC6749950.14148486722196638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)