FastQCFastQC Report
Sat 4 Feb 2023
EGAF00007889291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007889291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20399930
Sequences flagged as poor quality0
Sequence length91
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTTCACGTAATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4478732.195463415805839No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3304901.6200545786186522No Hit
ACAGCGCCGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2875461.4095440523570424No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2641501.294857384314554No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2508261.2295434347078642No Hit
ACAGCGCCGTATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2399171.176067761016827No Hit
ATGGTTCACGTAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2248751.1023322138850478No Hit
CTTATTTCCTGCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1799460.88209126207786No Hit
ATGGTTCACGTAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1681410.8242234164528995No Hit
ACTGATGGACTCAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1195060.5858157356422301No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1171590.5743107942037056No Hit
CGTGTTTGTTCCTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1079440.5291390705752421No Hit
CGTGTTTGTTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1032810.5062811490039427No Hit
ACTGATGGACTCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA987540.48408989638689937No Hit
GTGTGTTGTCCTATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA913610.44784957595442726No Hit
ACTGATGGACTCAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA913580.4478348700216128No Hit
TTTGTCCTGTACGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA896100.4392662131683785No Hit
GTGTGTTGTCCTATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA866920.42496224251749887No Hit
ACCAATCCTTTCGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA841440.4124720035804045No Hit
TCTCAGTGTTTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA836380.4099916029123629No Hit
CTTATTTCCTGCCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA829770.4067513957155735No Hit
CGTGTTTGTTCCTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA829670.4067023759395253No Hit
TTTGTCCTGTACGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA746970.36616302114762156No Hit
ACCAATCCTTTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA691830.33913351663461594No Hit
TGTGTATTCCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690880.3386678287621575No Hit
ACCAATCCTTTCGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA689270.33787861036778066No Hit
TCTCAGTGTTTGTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA649490.31837854345578637No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA633050.3103196922734539No Hit
TCTCAGTGTTTGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA631710.3096628272744073No Hit
TGGATTCCCGGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA613840.30090299329458486No Hit
GAGTTCCCTTACCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA606560.29733435359827215No Hit
TGTGTATTCCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA572770.28077057127156807No Hit
GAGTTCCCTTACCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA563480.27621663407668556No Hit
CTTATTTCCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA545510.26740778032081486No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA542930.26614307009877No Hit
AGACTAATAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA525320.25751068753667294No Hit
CTTATTTCCTGCCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA522100.25593225074791925No Hit
TGGATTCCCGGACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA515170.25253518026777544No Hit
CGTGTTTGTTCCTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA507130.24859399027349605No Hit
GAGTTCCCTTACCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA475640.23315766279590175No Hit
GTGTGTTGTCCTATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA465700.22828509705670558No Hit
TTTGTCCTGTACGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA445360.21831447460849132No Hit
AGTTCAGTCAACCGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA434460.21297131901923194No Hit
TGTTTACGAGAGCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA432220.21187327603575112No Hit
AGACTAATAGCTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426890.2092605219723793No Hit
GCTTTCTTCAGTATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410810.20137814198382056No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA397610.19490753154545137No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA387900.1901477112911662No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA385410.18892711886756475No Hit
CTTATTTCCTGCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA373760.1832163149579435No Hit
TGTTTACGAGAGCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA363750.17830943537551355No Hit
TGGATTCCCGGACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA356010.1745153047093789No Hit
TGTGTATTCCCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA354960.17400059706087226No Hit
CTTATTTCCTGCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA352970.17302510351751207No Hit
AGTTCAGTCAACCGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA352060.172579023555473No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA349910.17152509837043559No Hit
ATGGTTCACGTAATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT345860.16953979744048142No Hit
TCCTTGGATGTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA341210.16726037785423775No Hit
AGTTCAGTCAACCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA335710.16456429017158392No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT327450.16051525666999836No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318320.15603975111679305No Hit
CTTATTTCCTGCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA312410.1531426823523414No Hit
CATTAACGGGATGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311560.15272601425593127No Hit
GCTTTCTTCAGTATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA309460.15169659895891802No Hit
TGGATTCCCGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA285390.13989753886410394No Hit
AGACTAATAGCTGACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280560.1375298836809734No Hit
GACCTCATTGTGAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278460.13650046838396016No Hit
GCTTTCTTCAGTATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA276910.13574066185521225No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT270220.13246123883758426No Hit
GATCCCTTTGTCACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA268370.13155437298069161No Hit
GATCCCTTTGTCACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250420.12275532318003052No Hit
AAGTTCACTCTTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248870.12199551665128264No Hit
GCGATGGTAGATTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA244850.12002492165414294No Hit
TCCTTGGATGTACCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242600.11892197669305729No Hit
ACAGCGCCGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT241990.11862295605916295No Hit
CATTAACGGGATGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA241960.11860825012634847No Hit
TCCTTGGATGTACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237220.11628471274166138No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC234920.11515725789255159No Hit
CATTAACGGGATGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA233180.11430431378931201No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT231310.11338764397720973No Hit
TCCACTCATTCTGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA225190.11038763368305675No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223950.10977978846005844No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218850.10727977988159763No Hit
TCCTTTCCTGATAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217150.10644644368877736No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA215210.10549546003344129No Hit
TGGATTCCCGGACTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA214850.10531898883966759No Hit
TCCTTTCCTGATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212720.10427486760983984No Hit
TGTTTACGAGAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211590.10372094414049461No Hit
ACAGCGCCGTATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT209010.10245623391844973No Hit
GCGATGGTAGATTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205930.10094642481616359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)