FastQCFastQC Report
Sat 4 Feb 2023
EGAF00007889297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007889297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75089308
Sequences flagged as poor quality0
Sequence length91
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13663081.8195772958781296No Hit
ACAGCGCCGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11782351.5691115438165977No Hit
ACAGCGCCGTATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9815511.3071781138268048No Hit
ACCAATCCTTTCGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9106261.2127239206945415No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8585941.1434304335312293No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8584881.1432892682936964No Hit
GCTTTCTTCAGTATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7758641.0332549608793837No Hit
ACCAATCCTTTCGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7514041.0006804164449086No Hit
ACCAATCCTTTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7336030.9769739787720509No Hit
GCTTTCTTCAGTATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6673480.888739046576378No Hit
ACTGATGGACTCAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6100670.8124552166601403No Hit
GCTTTCTTCAGTATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6064400.8076249683909724No Hit
TTTGTCCTGTACGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5151330.6860270972266784No Hit
ACTGATGGACTCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5092710.678220393241605No Hit
TCTCAGTGTTTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890060.6512325296698699No Hit
ACTGATGGACTCAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4511840.6008631748211077No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4331070.576789174831655No Hit
TTTGTCCTGTACGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4109680.5473056164001405No Hit
CTTATTTCCTGCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3861850.514300917515447No Hit
TCTCAGTGTTTGTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3786000.5041996125467024No Hit
TCTCAGTGTTTGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3676170.489573029491762No Hit
GCGATGGTAGATTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3300360.43952462579625845No Hit
GTGTGTTGTCCTATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3275630.4362312141696658No Hit
GTACGCAGTCCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3196870.4257423706714676No Hit
GTACGCAGTCCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3142920.4185575927800533No Hit
GTGTGTTGTCCTATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3060660.40760263764849186No Hit
TCCTTGGATGTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3006500.4003898930590758No Hit
ACCTTTCGACACTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2881990.3838083046390573No Hit
GCTTAGCTGTATCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2575210.34295295410100196No Hit
TTTGTCCTGTACGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2563350.3413735015376623No Hit
CGTGTTTGTTCCTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2401280.31978986941789367No Hit
GCTTAGCTGTATCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2392130.31857132043352965No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2377570.31663229603873827No Hit
ACCTTTCGACACTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2312730.30799724509380216No Hit
GCGATGGTAGATTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2289590.3049155813235088No Hit
ATGGTTCACGTAATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2279000.3035052606957038No Hit
GCTTAGCTGTATCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2278680.3034626447749392No Hit
GCGATGGTAGATTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2244970.2989733238718887No Hit
TCCTTGGATGTACCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2194210.2922133734405969No Hit
CGTGTTTGTTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170580.28906645404163267No Hit
GTTGTCCGACAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2068460.2754666483276154No Hit
CGTGTTTGTTCCTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1981780.2639230607904923No Hit
TCCTTGGATGTACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1923190.2561203520479906No Hit
TGGATTCCCGGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1875240.24973462267091343No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1864640.24832297029558453No Hit
CTTATTTCCTGCCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1852080.24665029540557226No Hit
GTGTGTTGTCCTATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1738520.23152697052421897No Hit
GTACGCAGTCCTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1679920.22372293003419344No Hit
GATCCCTTTGTCACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1662860.2214509687584283No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1564630.20836921283120627No Hit
TGTTTACGAGAGCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1561020.20788845197508016No Hit
TGGATTCCCGGACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1548490.2062197723276395No Hit
AGTATCGTCTCTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1509870.20107656339035646No Hit
ACCTTTCGACACTCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1487530.19810143942197467No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1413800.18828246492829578No Hit
TGTTTACGAGAGCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1397750.18614501015244408No Hit
GATCCCTTTGTCACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1397490.18611038471682279No Hit
AGTATCGTCTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1358270.18088727092810603No Hit
AGCTTACCTGCACGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1339940.17844617771680624No Hit
TGTGTATTCCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1339560.1783955713108982No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1335840.17790016123200922No Hit
CCATTCAACTTCCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1268560.16894016389124267No Hit
AGCTTACCTGCACGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1267450.16879233991609033No Hit
ATGGTTCACGTAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1243620.1656187855666482No Hit
CTTATTTCCTGCCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1223820.1629819254693358No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1221500.16267296004379211No Hit
CTTATTTCCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1196970.15940618336767734No Hit
AGTATCGTCTCTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1176070.15662283104273647No Hit
CCATTCAACTTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1159670.15443876510354843No Hit
CGTGTTTGTTCCTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1138310.1515941523925084No Hit
TGGATTCCCGGACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1123540.14962716129971526No Hit
TGTGTATTCCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1085910.14461579536729782No Hit
GAGTTCCCTTACCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1083920.1443507776100427No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1076670.14338526065521873No Hit
AGACTAATAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1044760.13913565430646932No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1043650.13898783033131695No Hit
GAGTTCCCTTACCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1032100.13744966194121805No Hit
CCATTCAACTTCCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1009150.1343933013738787No Hit
GATCCCTTTGTCACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA998880.13302559666683839No Hit
ACAGCGCCGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT971810.1294205561196542No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA942690.12554250733007155No Hit
GAGTTCCCTTACCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA926900.12343967798984111No Hit
TCCACTCATTCTGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA919110.1224022466687268No Hit
TGGATTCCCGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA914300.12176167610973322No Hit
ATGGTTCACGTAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA908310.12096395934292004No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC891010.11866003612658142No Hit
GTCCCTGCAACTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA879050.11706726608800284No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT856780.11410146435228835No Hit
AGCTTACCTGCACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA855510.11393233241675366No Hit
TCCACTCATTCTGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA845240.11256462770971336No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA836710.11142864707183078No Hit
TCGCTTGGATGGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA829690.1104937603100564No Hit
ACAGCGCCGTATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT825740.1099677200381178No Hit
CTTATTTCCTGCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA823720.109698707038291No Hit
AGACTAATAGCTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA817290.10884239338042642No Hit
TGTTTACGAGAGCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA788510.10500962400665619No Hit
CTTATTTCCTGCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA781880.10412667539831369No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT759500.10114622443983635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)