FastQCFastQC Report
Fri 3 Feb 2023
EGAF00007889299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007889299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523290
Sequences flagged as poor quality0
Sequence length91
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161518645.8431182218892No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA631621.7926994371737777No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA511481.451711326629372No Hit
GGTTGCCAGATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA383621.0888118775349176No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA379641.0775156175052294No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA287530.8160838307377479No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA262640.745439631707864No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254890.7234431454691496No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252040.7153541150458804No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248860.7063284600472853No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189880.5389281041299467No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152840.4337990911903363No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148630.4218500322142089No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119570.3393703044597521No Hit
TGTCTTTCCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118190.3354535107811165No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112560.31947412787479884No Hit
AGTAAGTTCAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107900.30624785356868156No Hit
GGTTGCCAGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101530.28816816100860276No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86100.2443738664713946No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68500.1944205557873465No Hit
TGTCTTTCCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64670.18355003420098828No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT60180.1708062634639783No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55540.1576367542836383No Hit
GGTTGCCAGATGTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51280.14554578249306757No Hit
TTCCGCCTCTCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49010.1391029407173409No Hit
AAGTATCGTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48960.13896102790289758No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT48930.13887588021423158No Hit
AGTAAGTTCAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45560.12931095652075192No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC42330.12014338870771353No Hit
AAGTATCGTTTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40830.11588600427441396No Hit
AGTAAGTTCAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39540.11222465366177636No Hit
AGTAAGTTCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37880.10751314822225817No Hit
GGTTGCCAGATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT37030.10510063037672175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)