FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007889509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007889509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48772695
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG17696303.628321133371859TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCCCGTATG1205970.24726335093847082TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTATG934160.19153339793915428TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT715340.14666813060053377TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)