FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007889511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007889511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48757150
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG18173173.7272830754053508TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCCCGTATG1083510.22222586841109457TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTATG922480.18919891749210116TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT715120.14666977048494426TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)