FastQCFastQC Report
Sun 5 Feb 2023
EGAF00007890747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007890747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145804783
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4494620.308262864051586Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTC3955110.27126064856185134No Hit
CTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCG3133010.21487703870455332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2840150.19479127786912176Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA2522200.17298472300459444No Hit
CTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATC2476270.16983462058305726No Hit
CAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTG2325460.15949133849744834No Hit
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC2307290.1582451516696815No Hit
GTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGA2303920.15801402070602855No Hit
CCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCT2156720.14791832994943657No Hit
GCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACT2081380.14275114692225152No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC2025500.13891862518666484No Hit
AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGG2008100.1377252486977742No Hit
GACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGG1993820.13674585695861569No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1941990.13319110388854666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTG1938900.13297917668448503No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG1889120.12956502256856692No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC1728480.11854755135159044No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC1708300.11716350896390003No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT1701310.11668410082267329No Hit
GAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCA1619870.11109855017581967No Hit
CTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATT1615200.11077825889977834No Hit
CTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGT1566590.10744434906500976No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1562300.1071501200341281No Hit
AATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGA1528220.10481274815243888No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG1526120.1046687199555038No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG1487470.10201791528334156No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC1472610.10099874432788669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)