FastQCFastQC Report
Sun 5 Feb 2023
EGAF00007890751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007890751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146517308
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4688360.31998676907167856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTC3918360.26743325095762743No Hit
CTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCG3128200.21350378618749943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2902050.1980687496660804Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA2526540.17243969565698No Hit
CTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATC2469520.16854800526365118No Hit
CAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTG2320570.15838197081808245No Hit
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC2314770.15798611314917144No Hit
GTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGA2302110.15712205140978977No Hit
CCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCT2155090.1470877420161173No Hit
GCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACT2059710.14057793090219758No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC2050100.13992203569560532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2014020.1374595279896898Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGG1989270.13577030776459528No Hit
GACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGG1978060.13500521044244138No Hit
CTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTG1943180.1326246043231971No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG1869860.1276204173775838No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC1732300.1182317654921697No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC1721590.11750079383112881No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT1717950.11725235901822602No Hit
GAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCA1625700.1109561745428738No Hit
CTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATT1600820.1092580816458899No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1581940.10796949668226227No Hit
CTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGT1541680.10522169844944189No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG1531010.10449345684129005No Hit
AATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGA1519820.10372972454558065No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG1488600.10159891826568367No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC1484500.10131908784455691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)