FastQCFastQC Report
Sun 5 Feb 2023
EGAF00007890759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007890759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146057823
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4634750.31732295503267904Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTC3922900.26858540812291853No Hit
CTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCG3113610.21317653077712928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2869000.19642905399185634Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA2507430.17167378977023365No Hit
CTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATC2464000.16870030987658907No Hit
CAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTG2295020.15713091930721163No Hit
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC2294500.15709531696908832No Hit
GTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGA2278480.1559984910907511No Hit
CCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCT2144440.14682130377912042No Hit
GCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACT2060960.14110575918963272No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC2019200.13824661757419185No Hit
AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGG1993010.13645349212140456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1989280.13619811381140468Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGG1976640.13533270313086893No Hit
CTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTG1924170.1317402902821576No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG1869250.12798013564805769No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC1719190.11770612245808976No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT1702220.11654425384664265No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC1698360.11627997495211194No Hit
GAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCA1608830.11015021085176656No Hit
CTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATT1597760.10939229184594924No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1560800.10686178719780041No Hit
CTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGT1558460.10670157667624555No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG1519700.10404783316536219No Hit
AATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGA1514340.10368085521855273No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG1480210.10134410944903649No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC1470210.10065944910051138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)