FastQCFastQC Report
Thu 1 Jun 2023
EGAF00007893509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5092567
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110420.21682581692101446No Hit
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCG63560.12480935449646513Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCG57650.11320420526622428Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCG57480.11287038540680958Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCG56790.11151546950683221Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCG54060.10615471529387831Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAACGTCG51180.10049941414614673Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)