FastQCFastQC Report
Wed 8 May 2024
EGAF00007893510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12810900
Total Bases973.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCAGA141730.1106323521376328TruSeq Adapter, Index 18 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCAGA136040.1061908218782443TruSeq Adapter, Index 18 (97% over 37bp)
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCAGA131180.10239717740361723TruSeq Adapter, Index 18 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)