FastQCFastQC Report
Wed 8 May 2024
EGAF00007893572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5285245
Total Bases401.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCG62730.11868891602943668TruSeq Adapter, Index 1 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCG57420.10864207808720316TruSeq Adapter, Index 1 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCG57050.10794201593303622TruSeq Adapter, Index 1 (97% over 37bp)
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCG54710.10351459582289942TruSeq Adapter, Index 1 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCG53580.10137656816287609TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)