FastQCFastQC Report
Wed 8 May 2024
EGAF00007893573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5285245
Total Bases401.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119540.22617683759220245No Hit
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCCACT62320.11791317148022466Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCCACT56920.10769604814913972Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCCACT56600.10709058898877913Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCCACT54410.10294697786006135Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)