FastQCFastQC Report
Wed 8 May 2024
EGAF00007893576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15337607
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAA254870.16617324984269058TruSeq Adapter, Index 7 (97% over 35bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAA249060.1623851752101876TruSeq Adapter, Index 7 (97% over 35bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAA245880.16031184004127894TruSeq Adapter, Index 7 (97% over 35bp)
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAA243720.15890353690768058TruSeq Adapter, Index 7 (97% over 35bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAA233560.1522792962422365TruSeq Adapter, Index 7 (97% over 35bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAA215460.1404782375764355TruSeq Adapter, Index 7 (97% over 35bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAG198330.12930961133637078TruSeq Adapter, Index 7 (97% over 35bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAG192150.12528029959301995TruSeq Adapter, Index 7 (97% over 35bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAA183570.1196862065901154TruSeq Adapter, Index 7 (97% over 35bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAA162430.10590309166221301TruSeq Adapter, Index 7 (97% over 35bp)
CTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAG153670.10019164006484192TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)