FastQCFastQC Report
Thu 1 Jun 2023
EGAF00007893576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15337607
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAGATCTCGTATGCCGTCTTCTGCTTG210520.13725739615052074TruSeq Adapter, Index 7 (97% over 35bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAGATCTCGTATGCCGTCTTCTGCTTG196220.12793390781234648TruSeq Adapter, Index 7 (97% over 35bp)
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAGATCTCGTATGCCGTCTTCTGCTTG189490.12354600036368125TruSeq Adapter, Index 7 (97% over 35bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAGATCTCGTATGCCGTCTTCTGCTTG184030.11998612299819653TruSeq Adapter, Index 7 (97% over 35bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAGATCTCGTATGCCGTCTTCTGCTTG182860.11922329213416409TruSeq Adapter, Index 7 (97% over 35bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGGAAGATCTCGTATGCCGTCTTCTGCTTG171660.11192097958958004TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)