FastQCFastQC Report
Wed 8 May 2024
EGAF00007893577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15337607
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG486050.31690080466920295No Hit
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCAT225210.1468351614433725Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCAT215450.14047171765452068Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCAT212570.1385939801430562Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCAT198600.12948564922807057Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCAT195320.12734711484001382Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCAT188950.12319392458028165Illumina Single End Sequencing Primer (100% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCAT167010.10888921589919472Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCATG164360.10716143659177081Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCATG162190.10574661353625764Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)