FastQCFastQC Report
Wed 8 May 2024
EGAF00007893586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12919446
Total Bases981.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG302170.23388773791074322TruSeq Adapter, Index 16 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG294980.2283224837969058TruSeq Adapter, Index 16 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG278470.21554329806401915TruSeq Adapter, Index 16 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA271130.20986193990051893TruSeq Adapter, Index 16 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG263750.20414962065710868TruSeq Adapter, Index 16 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG249810.1933596843084448TruSeq Adapter, Index 16 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA249240.19291848891972613TruSeq Adapter, Index 16 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG245610.1901087709178861TruSeq Adapter, Index 16 (97% over 37bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA226750.17551062174028206TruSeq Adapter, Index 16 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG224950.17411737314432832TruSeq Adapter, Index 16 (97% over 37bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG202920.1570655583838502TruSeq Adapter, Index 16 (97% over 37bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG197150.15259942260682074TruSeq Adapter, Index 16 (97% over 37bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG196620.15218918829801215TruSeq Adapter, Index 16 (97% over 37bp)
GTTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA193680.14991354892462108TruSeq Adapter, Index 16 (97% over 37bp)
TTAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG183720.14220424002701046TruSeq Adapter, Index 16 (97% over 37bp)
GTTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG179760.13913909311591224TruSeq Adapter, Index 16 (97% over 37bp)
GAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA166560.12892193674558491TruSeq Adapter, Index 16 (97% over 37bp)
CTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA163940.12689398601147447TruSeq Adapter, Index 16 (97% over 37bp)
TAAGTCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG158940.12302385102271414TruSeq Adapter, Index 16 (97% over 37bp)
TAAGTCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG158110.12238140861457993TruSeq Adapter, Index 16 (97% over 37bp)
AAAGTCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG154020.11921563819377394TruSeq Adapter, Index 16 (97% over 37bp)
GTAGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG151780.11748181771880929TruSeq Adapter, Index 16 (97% over 37bp)
TAAGTCACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG149230.11550804887454152TruSeq Adapter, Index 16 (97% over 37bp)
GAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA147730.11434700837791342TruSeq Adapter, Index 16 (97% over 37bp)
CGTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG147540.11419994324834053TruSeq Adapter, Index 16 (97% over 37bp)
TAAGTCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA143170.11081744526816398TruSeq Adapter, Index 16 (97% over 37bp)
CCTGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA143090.1107555231083438TruSeq Adapter, Index 16 (97% over 37bp)
AAGTCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA141440.10947837856205289TruSeq Adapter, Index 16 (97% over 37bp)
CTTCTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG139620.10806964942614411TruSeq Adapter, Index 16 (97% over 37bp)
GGTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG138810.10744268755796495TruSeq Adapter, Index 16 (97% over 37bp)
CCGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA137460.10639775111099965TruSeq Adapter, Index 16 (97% over 37bp)
AAAGTCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG137190.10618876382160661TruSeq Adapter, Index 16 (97% over 37bp)
AAAGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG137170.10617328328165156TruSeq Adapter, Index 16 (97% over 37bp)
GTAGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG136140.10537603547396693TruSeq Adapter, Index 16 (97% over 37bp)
TAAGTCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG135120.10458652793625982TruSeq Adapter, Index 16 (97% over 37bp)
CCTGTCATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG134180.10385894255837286TruSeq Adapter, Index 16 (97% over 37bp)
AAGTCTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA134130.10382024120848526TruSeq Adapter, Index 16 (97% over 37bp)
AATGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG133720.10350289013940692TruSeq Adapter, Index 16 (97% over 37bp)
AATGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG133040.10297655178093551TruSeq Adapter, Index 16 (97% over 37bp)
AAGTCACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAGA132690.10270564233172227TruSeq Adapter, Index 16 (97% over 37bp)
GTAGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG132450.10251987585226177TruSeq Adapter, Index 16 (97% over 37bp)
TAAGTGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG130960.10136657562561119TruSeq Adapter, Index 16 (97% over 37bp)
ATACTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG130820.1012582118459259TruSeq Adapter, Index 16 (97% over 37bp)
TTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG130780.10122725076601581TruSeq Adapter, Index 16 (97% over 37bp)
AAAGTCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG130650.10112662725630804TruSeq Adapter, Index 16 (97% over 37bp)
TAAGTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG130210.10078605537729715TruSeq Adapter, Index 16 (97% over 37bp)
CCTGTCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG130170.10075509429738705TruSeq Adapter, Index 16 (97% over 37bp)
TATGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTAAG130010.10063124997774674TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)