FastQCFastQC Report
Wed 31 May 2023
EGAF00007893587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12919446
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA314760.2436327378124418Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA301250.23317563307281133Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290480.22483936230702153No Hit
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA289480.22406533530926945Illumina Single End PCR Primer 1 (97% over 36bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG277090.2144751408071213Illumina Single End PCR Primer 1 (97% over 36bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA266710.2064407405704548Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA258290.19992343324938236Illumina Single End PCR Primer 1 (97% over 36bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG254730.197167897137385Illumina Single End PCR Primer 1 (97% over 36bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA248940.1926862808204005Illumina Single End PCR Primer 1 (97% over 36bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA236960.1834134373873307Illumina Single End PCR Primer 1 (97% over 36bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG235890.182585228499736Illumina Single End PCR Primer 1 (97% over 36bp)
TTAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA208430.1613304471414641Illumina Single End PCR Primer 1 (97% over 36bp)
AAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA208080.1610595376922509Illumina Single End PCR Primer 1 (97% over 36bp)
CCTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA205280.1588922620985451Illumina Single End PCR Primer 1 (97% over 36bp)
AAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG202030.15637667435585087Illumina Single End PCR Primer 1 (97% over 36bp)
GTTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA191340.14810232574988125Illumina Single End PCR Primer 1 (97% over 36bp)
TTAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA185780.14379873564237972Illumina Single End PCR Primer 1 (97% over 36bp)
CCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG171200.1325134220151545Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGACAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG170370.1318709796070203Illumina Single End PCR Primer 1 (97% over 36bp)
GGTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA167480.12963404158351682Illumina Single End PCR Primer 1 (97% over 36bp)
GTAGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA164340.1272035968105753Illumina Single End PCR Primer 1 (97% over 36bp)
GTAGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA156560.1211816667680642Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGACTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA155960.12071725056941296Illumina Single End PCR Primer 1 (97% over 36bp)
CGTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA154780.11980389871206552Illumina Single End PCR Primer 1 (97% over 36bp)
GTTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG151650.11738119420910154Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGACACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA151440.11721864853957359Illumina Single End PCR Primer 1 (97% over 36bp)
CTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG149230.11550804887454152Illumina Single End PCR Primer 1 (97% over 36bp)
GAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG146800.11362716327000398Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGAGAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA145070.11228809656389292Illumina Single End PCR Primer 1 (97% over 36bp)
GGTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG144590.1119165636049719Illumina Single End PCR Primer 1 (97% over 36bp)
AAAGACTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA143440.11102643255755704Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGACACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA142630.11039947068937786Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGACGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG141230.10931583289252496Illumina Single End PCR Primer 1 (97% over 36bp)
TTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA141110.1092229496527947Illumina Single End PCR Primer 1 (97% over 36bp)
GGTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA140390.10866565021441321Illumina Single End PCR Primer 1 (97% over 36bp)
TTAGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA139040.10762071376744793Illumina Single End PCR Primer 1 (97% over 36bp)
TATGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA137690.10657577732048262Illumina Single End PCR Primer 1 (97% over 36bp)
CCTGACATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA137400.10635130949113454Illumina Single End PCR Primer 1 (97% over 36bp)
TAAGACAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA137210.10620424436156163Illumina Single End PCR Primer 1 (97% over 36bp)
GTTGACATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA136960.10601073761212362Illumina Single End PCR Primer 1 (97% over 36bp)
GTAGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG136840.10591785437239337Illumina Single End PCR Primer 1 (97% over 36bp)
TTAGACTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA136550.10569338654304528Illumina Single End PCR Primer 1 (97% over 36bp)
GTTGAGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA136440.10560824357329253Illumina Single End PCR Primer 1 (97% over 36bp)
CGTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG136280.10548439925365222Illumina Single End PCR Primer 1 (97% over 36bp)
CCTGACATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA136050.10530637304416923Illumina Single End PCR Primer 1 (97% over 36bp)
GGAGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA135980.10525219115432659Illumina Single End PCR Primer 1 (97% over 36bp)
CTTCACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA135640.10498902197509088Illumina Single End PCR Primer 1 (97% over 36bp)
AATGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA135340.10475681387576526Illumina Single End PCR Primer 1 (97% over 36bp)
GTTGACATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA132910.10287592827122774Illumina Single End PCR Primer 1 (97% over 36bp)
AATGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA131340.10166070588475697Illumina Single End PCR Primer 1 (97% over 36bp)
GTAGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA130890.10131239373576854Illumina Single End PCR Primer 1 (97% over 36bp)
TTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCA130670.10114210779626309Illumina Single End PCR Primer 1 (97% over 36bp)
AAAGACAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGTCCAG130220.10079379564727467Illumina Single End PCR Primer 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)