FastQCFastQC Report
Thu 9 May 2024
EGAF00007893592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3595610
Total Bases273.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCTCGTAT82270.22880679495273404TruSeq Adapter, Index 8 (97% over 39bp)
TAAGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCT78980.219656748090032TruSeq Adapter, Index 8 (97% over 39bp)
AAAGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCT59660.1659245580026755TruSeq Adapter, Index 8 (97% over 39bp)
TTAGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCT50200.1396146968108332TruSeq Adapter, Index 8 (97% over 39bp)
AATGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCT40550.11277641345974676TruSeq Adapter, Index 8 (97% over 39bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGAT40510.11266516668937954TruSeq Adapter, Index 8 (97% over 39bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGAT39760.11057928974499459TruSeq Adapter, Index 8 (97% over 39bp)
CTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCT38740.10774249710063105TruSeq Adapter, Index 8 (97% over 39bp)
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGAT38490.10704720478583606TruSeq Adapter, Index 8 (97% over 39bp)
TATGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCT37490.10426603552665611TruSeq Adapter, Index 8 (97% over 39bp)
AAGTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCTC37460.10418260044888071TruSeq Adapter, Index 8 (97% over 39bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGAT36200.100678327182314TruSeq Adapter, Index 8 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)