FastQCFastQC Report
Wed 8 May 2024
EGAF00007893593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007893593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3595610
Total Bases273.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1275693.5479098122432635No Hit
TAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATGGAGTGTGTA42430.11800501166700503Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATGGAGT36660.10195766504153678Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATGGAGT36110.10042802194898778Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)