FastQCFastQC Report
Fri 10 May 2024
EGAF00007894070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268349
Total Bases245.1 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA59340.18155955805209295TruSeq Adapter, Index 20 (97% over 37bp)
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG57460.1758074183632164TruSeq Adapter, Index 20 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG56350.1724112082277627TruSeq Adapter, Index 20 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG53130.16255913918617626TruSeq Adapter, Index 20 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA52740.16136587616561146TruSeq Adapter, Index 20 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG51000.15604208730462996TruSeq Adapter, Index 20 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG50790.15539956106278735TruSeq Adapter, Index 20 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG42990.13153430065149102TruSeq Adapter, Index 20 (97% over 37bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA42540.13015745870468545TruSeq Adapter, Index 20 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG42090.1287806167578799TruSeq Adapter, Index 20 (97% over 37bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG39220.11999942478603111TruSeq Adapter, Index 20 (97% over 37bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG39170.11984644234749717TruSeq Adapter, Index 20 (97% over 37bp)
TTAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG36210.11078988198628727TruSeq Adapter, Index 20 (97% over 37bp)
CTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA35910.10987198735508355TruSeq Adapter, Index 20 (97% over 37bp)
GAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA35570.1088317067730527TruSeq Adapter, Index 20 (97% over 37bp)
GTTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA35370.1082197770189169TruSeq Adapter, Index 20 (97% over 37bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG35290.10797500511726257TruSeq Adapter, Index 20 (97% over 37bp)
GTTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG33310.10191690055131812TruSeq Adapter, Index 20 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)