FastQCFastQC Report
Thu 9 May 2024
EGAF00007894071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268349
Total Bases245.1 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80270.24559800682240485No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG55090.16855605077670716Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG54870.16788292804715776Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG50520.15457345589470403Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG50110.15331899989872563Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG49420.1512078422469571Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG48810.1493414564968429Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG45480.13915282609048177Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG41460.1268530380323521Illumina Single End Sequencing Primer (100% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG41200.12605752935197556Illumina Single End Sequencing Primer (100% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG41120.12581275745032125Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG38290.11715395142929963Illumina Single End Sequencing Primer (100% over 33bp)
TTAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG36800.11259507476098789Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG34960.1069653210229385Illumina Single End Sequencing Primer (100% over 33bp)
AAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG34430.1053437071744786Illumina Single End Sequencing Primer (100% over 33bp)
AAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG34240.1047623739080496Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG34150.10448700551868849Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG33370.10210047947755885Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)