FastQCFastQC Report
Thu 1 Jun 2023
EGAF00007894071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268349
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69740.21337990526715478No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT51260.1568375959850065Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTATC48250.14762805318526265Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT47040.14392587817274102Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT45850.140284896135633Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT45490.13918342257818855Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTATC42730.13073879197111446Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT41550.12712840642171322Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT38710.1184390039129848Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT38420.11755170576948788Illumina Single End PCR Primer 1 (96% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTATC37790.11562412704396012Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT35920.10990258384279035Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGGTGTAGATCTCGGTGGTCGCCGTAT34260.10482356688346318Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)