FastQCFastQC Report
Fri 10 May 2024
EGAF00007894072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8455702
Total Bases642.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG167600.19820944494023085TruSeq Adapter, Index 20 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA157470.18622936333375986TruSeq Adapter, Index 20 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG154480.18269328791388342TruSeq Adapter, Index 20 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG151200.1788142486572966TruSeq Adapter, Index 20 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG144410.1707841643425939TruSeq Adapter, Index 20 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA136300.16119300325389896TruSeq Adapter, Index 20 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG134890.1595254894271345TruSeq Adapter, Index 20 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG130720.15459390598202255TruSeq Adapter, Index 20 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG127980.1513534890420689TruSeq Adapter, Index 20 (97% over 37bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA124420.14714331228796854TruSeq Adapter, Index 20 (97% over 37bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG113080.13373224363867128TruSeq Adapter, Index 20 (97% over 37bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG111930.1323722146310265TruSeq Adapter, Index 20 (97% over 37bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG107650.12731054145474852TruSeq Adapter, Index 20 (97% over 37bp)
GTTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA106810.12631712896220798TruSeq Adapter, Index 20 (97% over 37bp)
TTAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG103030.12184677274577557TruSeq Adapter, Index 20 (97% over 37bp)
CTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA99110.1172108477805864TruSeq Adapter, Index 20 (97% over 37bp)
GAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGA98100.11601638752169838TruSeq Adapter, Index 20 (97% over 37bp)
GTTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAG97540.11535411252667135TruSeq Adapter, Index 20 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)