FastQCFastQC Report
Thu 1 Jun 2023
EGAF00007894073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8455702
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG246540.2915665665606475No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG160370.1896590017008641Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG149070.1762952384083545Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG148470.17558565805653983Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG145120.1716238344255746Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG135000.15965557915830053Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG128970.1525242966225631Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG127050.15025363949675616Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG126150.14918926896903417Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG124970.14779376094379862Illumina Single End PCR Primer 1 (96% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG110780.1310121856233817Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG108070.12780724770101878Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG108060.12779542136182187Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG105610.12489796825857864Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG99750.11796773348918871Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG95080.1124448330842312Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTG94440.11168794737562888Illumina Single End PCR Primer 1 (96% over 33bp)
AAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG94420.11166429469723507Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCACATTGG93430.11049348711674087Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)