FastQCFastQC Report
Wed 8 May 2024
EGAF00007894102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2982023
Total Bases223.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG49060.16451918714241975TruSeq Adapter, Index 1 (97% over 36bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG44520.14929462314676983TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG44100.14788618330576256TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC43460.14573998926232293TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG42900.14386206947431324TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG40270.13504255332705348TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC39440.13225920792696771TruSeq Adapter, Index 1 (97% over 36bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG36250.12156177199169826TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG33860.11354707861072837TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC31350.10512997384661352TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)