FastQCFastQC Report
Fri 10 May 2024
EGAF00007894104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21119236
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG381950.1808540801381262TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG353090.16718881308017014TruSeq Adapter, Index 1 (97% over 36bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG350700.16605714335499638TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC333510.1579176443693323TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG328480.15553592942471972TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG307520.14561132798553886TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC299960.14203165303896412TruSeq Adapter, Index 1 (97% over 36bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG299460.14179490205043402TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG275450.1304261195812197TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC253220.11990017063117245TruSeq Adapter, Index 1 (97% over 36bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG236480.11197374753518546TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG220880.10458711669304704TruSeq Adapter, Index 1 (97% over 36bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG218290.10336074657246125TruSeq Adapter, Index 1 (97% over 36bp)
GTTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC216960.10273098894297122TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)