FastQCFastQC Report
Wed 31 May 2023
EGAF00007894105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21119236
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG520850.24662350475178177No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG382880.18129443697679215Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG350890.1661471087306378Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG349820.16564046161518342Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGGG327820.15522341811986Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG320230.15162953811397342Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG298440.14131193003383266Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG297360.1408005478986077Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGGG295120.13973990346999296Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG278900.13205970140207723Illumina Single End PCR Primer 1 (96% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGGG255330.12089925980276939Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG239660.11347948382223676Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG219620.10399050420195124Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG219530.10394788902401582Illumina Single End PCR Primer 1 (96% over 33bp)
AAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGGG219070.10373007811456816Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)