FastQCFastQC Report
Wed 8 May 2024
EGAF00007894106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11379658
Total Bases864.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG189740.1667361180801743TruSeq Adapter, Index 1 (97% over 36bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG178600.15694672019141526TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG169310.14878303021057399TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC167020.14677066744888115TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG166820.14659491524261978TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG153020.1344680130105843TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC152890.13435377407651442TruSeq Adapter, Index 1 (97% over 36bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG141300.12416893372366726TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG132830.11672582778849767TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC125050.10988906696492988TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)