FastQCFastQC Report
Wed 8 May 2024
EGAF00007894108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13259419
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG230220.17362751716345942TruSeq Adapter, Index 1 (97% over 36bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG210740.15893607404668333TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG210360.15864948532058606TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG199590.15052695747830278TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC193340.14581332711486075TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG185860.14017205429589336TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC178480.134606199562741TruSeq Adapter, Index 1 (97% over 36bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG174970.13195902475063198TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG163680.12344432286211032TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC151310.11411510564678588TruSeq Adapter, Index 1 (97% over 36bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG135440.10214625542793392TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)