FastQCFastQC Report
Wed 31 May 2023
EGAF00007894112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5824707
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG99410.17066952895656382TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG91270.15669457708344814TruSeq Adapter, Index 1 (97% over 36bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG90070.15463438761812398TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG87140.1496040916736241TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC85490.14677133115880336TruSeq Adapter, Index 1 (97% over 36bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC80990.1390456206638377TruSeq Adapter, Index 1 (97% over 36bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG78870.13540595260843163TruSeq Adapter, Index 1 (97% over 36bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG72780.12495049107191143TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAG69480.11928497004226994TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGAGC64620.110941202707707TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)