FastQCFastQC Report
Wed 8 May 2024
EGAF00007894113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5824707
Total Bases442.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141850.24353156304686227No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG95810.16448896056059129Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG86200.14799027659245348Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG86180.14795594010136476Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGGG81070.13918296662819263Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG80060.13744897382821145Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGGG75380.12941423491344714Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG72120.12381738686598313Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG69950.12009187758285525Illumina Single End Sequencing Primer (100% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGG67710.11624619058091677Illumina Single End Sequencing Primer (100% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATCAGGG62600.10747321710774464Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)