FastQCFastQC Report
Wed 8 May 2024
EGAF00007894118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792700
Total Bases209.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT35560.127331972642962TruSeq Adapter, Index 12 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT33690.1206359437103878TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA33290.11920363805636124TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA31990.11454864468077489TruSeq Adapter, Index 12 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT31730.1136176460056576TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT31450.11261503204783901TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT30390.1088194220646686TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)