FastQCFastQC Report
Wed 8 May 2024
EGAF00007894120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19790693
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT286810.1449216558510609TruSeq Adapter, Index 12 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT269900.13637723550155623TruSeq Adapter, Index 12 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT263900.13334550740593065TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA258800.13076853852464893TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT247250.12493246194056973TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA239200.1208648934122721TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT235610.11905090943505617TruSeq Adapter, Index 12 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT230480.11645878191329632TruSeq Adapter, Index 12 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT205780.10397816791963778TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)