FastQCFastQC Report
Wed 8 May 2024
EGAF00007894122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8975554
Total Bases682.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT118190.1316798940767333TruSeq Adapter, Index 12 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT111050.1237249533566396TruSeq Adapter, Index 12 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT108840.12126270980042012TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA107760.12005944145620426TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT103750.11559175065962501TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA100790.11229390408658897TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT97180.10827186823231191TruSeq Adapter, Index 12 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT91950.10244492986171104TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)