FastQCFastQC Report
Wed 8 May 2024
EGAF00007894124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12829751
Total Bases975 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT165070.12866188907329534TruSeq Adapter, Index 12 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT154100.12011145033134314TruSeq Adapter, Index 12 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT152310.11871625567791613TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA151330.11795240609112367TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT142670.11120246994661082TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA142570.11112452611122382TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT137330.10704026913694585TruSeq Adapter, Index 12 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT128640.10026694984181687TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)