FastQCFastQC Report
Wed 8 May 2024
EGAF00007894128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5304128
Total Bases403.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT71380.13457442957636015TruSeq Adapter, Index 12 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT67430.12712739964043102TruSeq Adapter, Index 12 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT65850.12414858766605934TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA65710.12388464230124159TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT61950.11679582393185081TruSeq Adapter, Index 12 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTA60840.11470311425365301TruSeq Adapter, Index 12 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT59240.1116865957985931TruSeq Adapter, Index 12 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCT56070.10571011860950565TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)