FastQCFastQC Report
Wed 8 May 2024
EGAF00007894132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049396
Total Bases228.7 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT51440.1686891436861595TruSeq Adapter, Index 2 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT48380.1586543695866329TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT48340.15852319606899204TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT47660.15629324626909724TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT46870.15370256929569TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT44850.14707830665482607TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT44540.14606171189310932TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT38960.12776300618220787TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT38580.12651685776461963TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT36520.11976142160611478TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT33730.11061206875066408TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT32640.10703759039495035TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT31790.10425015314508183TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT30910.10136433575698268TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)