FastQCFastQC Report
Thu 9 May 2024
EGAF00007894133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049396
Total Bases228.7 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74000.24267100763561045No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG51110.16760696216562232Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG47920.15714587413376288Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG47120.15452240378094548Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG46960.15399770971038199Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG44230.14504511713139256Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG43130.14143784539626864Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG42310.1387487882846308Illumina Single End Sequencing Primer (100% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG38930.12766462604397724Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG38320.12566422989995396Illumina Single End Sequencing Primer (100% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG35680.11700677773565651Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG33690.1104808952330232Illumina Single End Sequencing Primer (100% over 33bp)
TTAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG32210.10562747508031098Illumina Single End Sequencing Primer (100% over 33bp)
AAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG32000.10493881411269641Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG31260.10251210403634031Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)