FastQCFastQC Report
Wed 8 May 2024
EGAF00007894134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18544911
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT348320.18782511277622202TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT328190.1769703828721529TruSeq Adapter, Index 2 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT321310.17326047021740898TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT314620.1696530115458629TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT309650.16697303103800282TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT287930.1552609230640147TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT287560.1550614074125241TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT279550.15074216317349812TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT259920.14015704901468656TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT247400.13340587075343743TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT227270.12255114084936831TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT226180.12196337852470687TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT225170.12141875471928659TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT213450.11509896165044955TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT210890.11371852903473087TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT200020.10785708273283166TruSeq Adapter, Index 2 (97% over 37bp)
CTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT195920.10564623362171972TruSeq Adapter, Index 2 (97% over 37bp)
GAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT188200.10148336651494311TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)