FastQCFastQC Report
Thu 1 Jun 2023
EGAF00007894135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18544911
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG475000.2561349579946757No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG350570.18903838362988098Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG327270.17647428990087902Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG322800.17406392513827648Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG314140.16939418043041565Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG304980.16445481997729727Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG285430.15391284433772695Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG281060.15155640272417592Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG279620.15077990937783417Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG265020.1429071296163136Illumina Single End PCR Primer 1 (96% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG250620.13514219615289605Illumina Single End PCR Primer 1 (96% over 33bp)
AAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG230830.12447080495560212Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG226720.12225456352958503Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG226530.12215210954638715Illumina Single End PCR Primer 1 (96% over 33bp)
AAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG216500.11674361769652061Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGACTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG213450.11509896165044955Illumina Single End PCR Primer 1 (96% over 33bp)
TTAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG200760.10825611403581284Illumina Single End PCR Primer 1 (96% over 33bp)
TAAGACAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG196500.10595898788621849Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG187430.10106815826724648Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)