FastQCFastQC Report
Thu 9 May 2024
EGAF00007894136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9642248
Total Bases732.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT169510.17579925345209954TruSeq Adapter, Index 2 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT157820.16367552462869653TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT154930.16067829825575944TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT154420.1601493759546529TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT151980.157618845729751TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT141960.14722707816683414TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT140230.14543289075327662TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT129490.13429440935350345TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT123170.12773992122998704TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT115940.12024166978488833TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT110560.11466205805948987TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT107380.11136407194670787TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT104030.10788977840022367TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT103910.10776532609408096TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT97670.10129380617465969TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)