FastQCFastQC Report
Wed 31 May 2023
EGAF00007894138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23256054
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT423520.18211172024282365TruSeq Adapter, Index 2 (97% over 37bp)
TAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT389650.16754777057191214TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT389380.16743167177028398TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT372980.16037974456027665TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT370460.1592961557450804TruSeq Adapter, Index 2 (97% over 37bp)
AAAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT349730.15038234775340648TruSeq Adapter, Index 2 (97% over 37bp)
AAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT343430.14767337571541586TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT323400.139060564616852TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT311270.1338447184548161TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT294690.1267153920437233TruSeq Adapter, Index 2 (97% over 37bp)
CCTGTCTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT271040.11654599701221884TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT265790.11428852031389332TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT261940.11263303740178794TruSeq Adapter, Index 2 (97% over 37bp)
TTAGTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGT247860.10657869989465969TruSeq Adapter, Index 2 (97% over 37bp)
GTTGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTT246720.10608850495445185TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)