FastQCFastQC Report
Sat 11 May 2024
EGAF00007894139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007894139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23256054
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG668230.28733593411848807No Hit
CCTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG339500.14598349315838363Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG312020.13416721512600546Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG301030.12944156390417738Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG290930.12509860873216067Illumina Single End Sequencing Primer (100% over 33bp)
CCTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG286010.12298303056915846Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG267540.11504101254666849Illumina Single End Sequencing Primer (100% over 33bp)
GTTGACTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG266040.11439601920428977Illumina Single End Sequencing Primer (100% over 33bp)
TAAGACAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG255610.10991116549694974Illumina Single End Sequencing Primer (100% over 33bp)
AAAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATG251640.10820408311745407Illumina Single End Sequencing Primer (100% over 33bp)
AAGACAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTGGTATGG239820.10312153557950975Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)